Methods to Make Homogenous Antibody Drug Conjugates
نویسندگان
چکیده
منابع مشابه
Methods to Design and Synthesize Antibody-Drug Conjugates (ADCs).
Antibody-drug conjugates (ADCs) have become a promising targeted therapy strategy that combines the specificity, favorable pharmacokinetics and biodistributions of antibodies with the destructive potential of highly potent drugs. One of the biggest challenges in the development of ADCs is the application of suitable linkers for conjugating drugs to antibodies. Recently, the design and synthesis...
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Background: It is believed that the loading value of anticancer drug conjugated to the monoclonal antibody, called drug-to-antibody ratio (DAR), is the main quality feature of antibody-drug conjugates. Methods: In this study, matrix assisted laser desorption/ionization mass spectrometry was used to determine the average molecular weight of trastuzumab and its three conjugated forms. The differe...
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Background: Reduction/alkylation is one of the leading strategies for the development of antibody drug conjugates (ADCs). Precise control of the reduction process would not only yield a defined number of free thiols per antibody but also result in development of more homogenous conjugates. Methods: In the present study, we investigated the effect of various dithiothreitol (DTT) concentrations, ...
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30 mAbs Volume 6 Issue 1 Antibody-drug conjugates (ADCs) represent an exciting and promising therapeutic approach for the treatment of cancers. The US Food and Drug Administration’s recent approval of brentuximab vedotin (Adcetris) in 2011 and trastuzumab emtansine (Kadcyla) in 2013 has brought a proof of the ADC concept in hematologic and solid tumors, respectively. The number of ADCs or immun...
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ژورنال
عنوان ژورنال: Pharmaceutical Research
سال: 2014
ISSN: 0724-8741,1573-904X
DOI: 10.1007/s11095-014-1596-8